FBM Facilities

The education within the FBM-initiative is carried out on state-of-the-art equipment, which is also widely used in the fermentation industry. It allows expert education in fermentation, which is directly applicable in most of the industrial companies working with fermentation. This facilitates the transition into industry careers or tailor-made skill-development for industry professionals.

The three involved DTU units have the extensive infrastructure available, to provide education in fermentation at the highest level. DTU Bioengineering hosts a large Lab-scale fermentation facility (fermentation core), a pre-pilot facility at DTU Biosustain, and a non-GMO pilot facility at DTU Chemical engineering.
Within the Fermentation Based Biomanufacturing initiative, an automation and high throughput micro-fermentation facility and a GMO class I Pilot facility, including a downstream facility, are currently added to the existing facilities.  

Once the constructions are completed, the infrastructure of the Fermentation Based Biomanufacturing initiative, will cover the entire fermentation chain from discovery and selection of target molecules to proof of concept in the pilot facility. In cell factory engineering and bioreactor engineering, this offers a unique educational environment worldwide.


Contact

Friederike Buchholz
Joint Program Manager
DTU Bioengineering
+4593 51 89 33

Fermentation Core

The fermentation core of DTU Bioengineering contains a training unit with a well-equipped lab-scale fermentation setup of 68 bioreactors at 1-10 liter scale, which guarantees a maximum of hands-on experience during the educational programs. Besides the education activities, the fermentation core supports the research in the department as well as contract projects with industry partners.

The fermentation core is the central unit for hands-on education in cell-factory engineering and fermentation technology within the FBM - educational programs. Besides courses taking place in the fermentation training labs, the facilities are open for student projects within the master study line, so students can use that opportunity to work on individual projects as part of their curriculum. Additionally, some of the FBM-PhD students are using the fermentation core facility as part of their projects.
 

Translational Core

The Translational Core at DTU Biosustain, also known as Pre-Pilot facility, is a Downstream Process Development Unit, where pilot scale-up, as well as purification and separation of the compound of interest from the fermentation broth is performed.

The Unit’s primary focus is to develop efficient purification processes. It includes improving existing methods and to design completely new downstream processes.

The expertise spans over various unit operations from laboratory scale to pilot scale. It includes solid-liquid separation, various filtration, liquid-liquid separation, evaporation, multiple chromatography columns, crystallization etc.

Pilot Facility

DTU Chemical Engineering currently has pilot fermentation facilities including conventional fermentors in the sizes of 80. 150 and 200 liters, as well as a 150 L U-loop fermenter. All can be steam sterilized in situ, but are presently only approved for operation with non-GMO microorganisms. A GMO 1 pilot facility is in construction to be commissioned 2020.

The pilot fermentation facilities are supported by conventional downstream unit operations, including centrifugation, separation, ultrafiltration, microfiltration, evaporation and crystallization. The pilot facilities are complemented by smaller scale non-GMO, autoclavable fermenters (2 x 5L, 2 x L, and 2 x 2L with fed-batch operation). Additionally, a number of smaller fermenters are also used in GMO approved facilities solely for research.